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标题：Factors influencing the utilization of ketone bodies by mouse adipose tissue
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作者：Richard W. Hanson ; Zigmund Z. Ziporin
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1966
卷号：7
期号：1
页码：56-61
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Factors influencing the utilization of ketone bodies by mouse adipose tissue in vitro were studied. Epididymal fat pads can oxidize dl -β-hydroxybutyrate-3-¹⁴C and acetoacetate-3-¹⁴C to ¹⁴CO₂ as well as convert these compounds to fatty acid-¹⁴C. An increased output of ¹⁴CO₂ from β-hydroxybutyrate-3-¹⁴C was noted in response to glucose plus insulin, succinate, oxaloacetate, l -asparate, and l -malate. Fatty acid synthesis from β-hydroxybutyrate was enhanced by glucose plus insulin, l -aspartate, l -malate, oxaloacetate, and citrate. Nicotinamide stimulated the oxidation of β-hydroxybutyrate but not of acetoacetate to CO₂, and did not affect fatty acid synthesis from either ketone body. Nicotinamide increased NAD⁺ and NADP⁺ levels in epididymal fat pads without affecting the concentration of NADH and NADPH. “Superlipogenesis” caused by fasting the mice for 48 hr and re-feeding them for 24 hr sharply enhanced CO₂ output and lipogenesis from β-hydroxybutyrate. The activities of glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase, NADP-malic dehydrogenase, and citrate cleavage enzyme from mouse adipose tissue were increased during “superlipogenesis.” Free fatty acid release by epididymal fat pads in vitro was slightly increased by β-hydroxybutyrate. The relationship of ketone body metabolism and lipogenesis in adipose tissue is discussed.
关键词：adipose tissue ; ketone bodies ; metabolism ; oxidation ; conversion to fatty acid ; lipogenesis ; enzymes ; NAD ; NADP ; nucleotide coenzymes ; nicotinamide ; free fatty acid release ; mouse