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标题：Adaptive changes in enzyme activity and metabolic pathways in adipose tissue from meal-fed rats
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作者：Gilbert A. Leveille ; Richard W. Hanson
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1966
卷号：7
期号：1
页码：46-55
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A number of metabolic factors and the activity of a number of enzymes were determined in meal-fed (animals fed a single daily 2 hr meal) and nibbling (ad libitum-fed) rats. The dependency of the observed adaptive changes on the ingestion of carbohydrate was studied by feeding diets high in carbohydrate or fat. Glucose-6-phosphate dehydrogenase and NADP-malic dehydrogenase were more active in adipose tissue from high carbohydrate meal-fed rats than in tissue from ad libitum-fed rats. The activity in adipose tissue of isocitric dehydrogenase, 6-phosphogluconate dehydrogenase, and NAD-malic dehydrogenase did not increase significantly in response to meal-feeding the high carbohydrate diet. No increase in lipogenesis or enzyme activity could be demonstrated in adipose tissue from rats meal-fed a high fat diet. Lipase activity of adipose tissue was increased by high carbohydrate meal-feeding and decreased by feeding a high fat diet. The in vitro uptake of palmitate-1-¹⁴C by adipose tissue was depressed by a high fat diet and enhanced in rats meal-fed a high carbohydrate diet. Diaphragm or slices of liver from high fat-fed rats oxidized palmitate-1-¹⁴C more rapidly than did tissue from ad libitumfed animals. Evidence is presented for the quantitative importance of citrate as a source of extramitochondrial acetyl CoA in adipose tissue of meal-eating and ad libitum-fed rats. The relationship of extramitochondrially formed citrate to the NAD-malic dehydrogenase–malic enzyme system in adipose tissue is discussed.
关键词：lipogenesis ; enzymes ; activity ; adaptations ; meal-feeding ; nibbling ; high fat ; high carbohydrate ; diet ; rat ; citrate ; pathways ; adipose tissue ; metabolism ; lipase activity ; palmitate utilization