文章基本信息

标题：Acetic anhydride–trifluoroacetic acid acetolysis for the estimation of glycerol in phosphatidyl choline by gas–liquid chromatography
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作者：K. S. Holla ; David G. Cornwell
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1965
卷号：6
期号：2
页码：322-324
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Low glycerol recoveries are obtained when acetic anhydride–acetic acid acetolysis is used to dephosphorylate lecithin prior to glycerol (and fatty acid) analysis by saponification–acetylation and gas–liquid chromatography. Quantitative glycerol recoveries are obtained for both phosphatidyl choline and phosphatidyl ethanolamine when a modified acetolysis using acetic anhydride–trifluoroacetic acid is employed. Modified acetolysis is limited to the analysis of glycerol alone since unsaturated fatty acids are destroyed.
关键词：glycerol estimation ; phosphatidyl choline phosphatidyl ethanolamine ; acetolysis ; trifluoroacetic acid ; glyceryl triacetate ; gas–liquid chromatography