文章基本信息

标题：Alcohol produces distinct hepatic lipidome and eicosanoid signature in lean and obese
本地全文：下载
作者：Puneet Puri ; Jun Xu ; Terhi Vihervaara 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2016
卷号：57
期号：6
页码：1017-1028
DOI：10.1194/jlr.M066175
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Alcohol- and obesity-related liver diseases often coexist. The hepatic lipidomics due to alcohol and obesity interaction is unknown. We characterized the hepatic lipidome due to 1 ) alcohol consumption in lean and obese mice and 2 ) obesity and alcohol interactions. In the French-Tsukamoto mouse model, intragastric alcohol or isocaloric dextrose were fed with either chow (lean) or high-fat, high-cholesterol diet (obese). Four groups (lean, lean alcohol, obese, and obese alcohol) were studied. MS was performed for hepatic lipidomics, and data were analyzed. Alcohol significantly increased hepatic cholesteryl esters and diacylglycerol in lean and obese but was more pronounced in obese. Alcohol produced contrasting changes in hepatic phospholipids with significant enrichment in lean mice versus significant decrease in obese mice, except phosphatidylglycerol, which was increased in both lean and obese alcohol groups. Most lysophospholipids were increased in lean alcohol and obese mice without alcohol use only. Prostaglandin E₂; 5-, 8-, and 11-hydroxyeicosatetraenoic acids; and 9- and 13-hydroxyoctadecadienoic acids were considerably increased in obese mice with alcohol use. Alcohol consumption produced distinct changes in lean and obese with profound effects of obesity and alcohol interaction on proinflammatory and oxidative stress-related eicosanoids.
关键词：cyclooxygenase ; cytochrome P450 ; hydroxyeicosatetraenoic acids ; lipoxygenase ; liver ; obesity ; phospholipids ; prostaglandins