文章基本信息

标题：Establishment of LC-MS methods for the analysis of palmitoylated surfactant proteins
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作者：Takeshi Harayama ; Hideo Shindou ; Yoshihiro Kita 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2015
卷号：56
期号：7
页码：1370-1379
DOI：10.1194/jlr.D060236
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The surfactant proteins (SPs), SP-B and SP-C, are important components of pulmonary surfactant involved in the reduction of alveolar surface tension. Quantification of SP-B and SP-C in surfactant drugs is informative for their quality control and the evaluation of their biological activity. Western blot analysis enabled the quantification of SP-B, but not SP-C, in surfactant drugs. Here, we report a new procedure involving chemical treatments and LC-MS to analyze SP-C peptides. The procedure enabled qualitative analysis of SP-C from different species with discrimination of the palmitoylation status and the artificial modifications that occur during handling and/or storage. In addition, the method can be used to estimate the total amount of SP-C in pulmonary surfactant drugs. The strategy described here might serve as a prototype to establish analytical methods for peptides that are extremely hydrophobic and behave like lipids. The new method provides an easy measurement of SP-C from various biological samples, which will help the characterization of various experimental animal models and the quality control of surfactant drugs, as well as diagnostics of human samples.
关键词：pulmonary surfactant ; mass spectrometry ; palmitoylation ; proteomics ; lung ; drug therapy ; surfactant drug ; liquid chromatography