文章基本信息

标题：Micellar lipid composition affects micelle interaction with class B scavenger receptor extracellular loops
本地全文：下载
作者：Aurélie Goncalves ; Brigitte Gontero ; Marion Nowicki 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2015
卷号：56
期号：6
页码：1123-1133
DOI：10.1194/jlr.M057612
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Scavenger receptors (SRs) like cluster determinant 36 (CD36) and SR class B type I (SR-BI) play a debated role in lipid transport across the intestinal brush border membrane. We used surface plasmon resonance to analyze real-time interactions between the extracellular protein loops and various ligands ranging from single lipid molecules to mixed micelles. Micelles mimicking physiological structures were necessary for optimal binding to both the extracellular loop of CD36 (lCD36) and the extracellular loop of SR-BI (lSR-BI). Cholesterol, phospholipid, and fatty acid micellar content significantly modulated micelle binding to and dissociation from the transporters. In particular, high phospholipid micellar concentrations inhibited micelle binding to both receptors (−53.8 and −74.4% binding at 0.32 mM compared with 0.04 mM for lCD36 and lSR-BI, respectively, P P
关键词：scavenger receptor class B type I ; cluster determinant 36 ; cholesterol ; phospholipids ; fatty acid ; cholecalciferol ; intestine ; surface plasmon resonance