文章基本信息

标题：A brief elevation of serum amyloid A is sufficient to increase atherosclerosis
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作者：Joel C. Thompson ; Colton Jayne ; Jennifer Thompson 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2015
卷号：56
期号：2
页码：286-293
DOI：10.1194/jlr.M054015
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Serum amyloid A (SAA) has a number of proatherogenic effects including induction of vascular proteoglycans. Chronically elevated SAA was recently shown to increase atherosclerosis in mice. The purpose of this study was to determine whether a brief increase in SAA similarly increased atherosclerosis in a murine model. The recombination activating gene 1-deficient ( rag1 ^−/−) × apolipoprotein E-deficient ( apoe ^−/−) and apoe ^−/− male mice were injected, multiple times or just once respectively, with an adenoviral vector encoding human SAA1 (ad-SAA); the injected mice and controls were maintained on chow for 12–16 weeks. Mice receiving multiple injections of ad-SAA, in which SAA elevation was sustained, had increased atherosclerosis compared with controls. Strikingly, mice receiving only a single injection of ad-SAA, in which SAA was only briefly elevated, also had increased atherosclerosis compared with controls. Using in vitro studies, we demonstrate that SAA treatment leads to increased LDL retention, and that prevention of transforming growth factor beta (TGF-β) signaling prevents SAA-induced increases in LDL retention and SAA-induced increases in vascular biglycan content. We propose that SAA increases atherosclerosis development via induction of TGF-β, increased vascular biglycan content, and increased LDL retention. These data suggest that even short-term inflammation with concomitant increase in SAA may increase the risk of developing CVD.
关键词：apolipoproteins ; extracellular matrix ; lipoproteins ; proteoglycans ; vascular biology ; free-form: biglycan ; transforming growth factor beta ; cardiovascular disease