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标题：Biohydrogenation of C20 polyunsaturated fatty acids by anaerobic bacteria
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作者：Haruko Sakurama ; Shigenobu Kishino ; Kousuke Mihara 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2014
卷号：55
期号：9
页码：1855-1863
DOI：10.1194/jlr.M045450
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The PUFAs include many bioactive lipids. The microbial metabolism of C₁₈ PUFAs is known to produce their bioactive isomers, such as conjugated FAs and hydroxy FAs, but there is little information on that of C₂₀ PUFAs. In this study, we aimed to obtain anaerobic bacteria with the ability to produce novel PUFAs from C₂₀ PUFAs. Through the screening of ∼100 strains of anaerobic bacteria, Clostridium bifermentans JCM 1386 was selected as a strain with the ability to saturate PUFAs during anaerobic cultivation. This strain converted arachidonic acid ( cis -5, cis -8, cis -11, cis -14-eicosatetraenoic acid) and EPA ( cis -5, cis -8, cis -11, cis -14, cis -17-EPA) into cis -5, cis -8, trans -13-eicosatrienoic acid and cis -5, cis -8, trans -13, cis -17-eicosatetraenoic acid, giving yields of 57% and 67% against the added PUFAs, respectively. This is the first report of the isolation of a bacterium transforming C₂₀ PUFAs into corresponding non-methylene-interrupted FAs. We further investigated the substrate specificity of the biohydrogenation by this strain and revealed that it can convert two cis double bonds at the ω6 and ω9 positions in various C₁₈ and C₂₀ PUFAs into a trans double bond at the ω7 position. This study should serve to open up the development of novel potentially bioactive PUFAs.
关键词：arachidonic acid ; fatty acid/metabolism ; omega-3 fatty acids ; lipids/chemistry ; diet and dietary lipids ; eicosapentaenoic acid ; conjugated fatty acid ; non-methylene-interrupted fatty acids