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标题：Characterization of acyl chain position in unsaturated phosphatidylcholines using differential mobility-mass spectrometry
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作者：Alan T. Maccarone ; Jackson Duldig ; Todd W. Mitchell 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2014
卷号：55
期号：8
页码：1668-1677
DOI：10.1194/jlr.M046995
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Glycerophospholipids (GPs) that differ in the relative position of the two fatty acyl chains on the glycerol backbone (i.e., sn -positional isomers) can have distinct physicochemical properties. The unambiguous assignment of acyl chain position to an individual GP represents a significant analytical challenge. Here we describe a workflow where phosphatidylcholines (PCs) are subjected to ESI for characterization by a combination of differential mobility spectrometry and MS (DMS-MS). When infused as a mixture, ions formed from silver adduction of each phospholipid isomer {e.g., [PC (16:0/18:1) + Ag]⁺ and [PC (18:1/16:0) + Ag]⁺} are transmitted through the DMS device at discrete compensation voltages. Varying their relative amounts allows facile and unambiguous assignment of the sn -positions of the fatty acyl chains for each isomer. Integration of the well-resolved ion populations provides a rapid method (
关键词：lipid isomers ; sn -positional isomers ; mass spectrometry ; differential mobility spectrometry