文章基本信息

标题：Lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes
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作者：Benjamin J. Reading ; Naoshi Hiramatsu ; Justin Schilling 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2014
卷号：55
期号：11
页码：2287-2295
DOI：10.1194/jlr.M050286
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Transcripts encoding a novel member of the lipoprotein receptor superfamily, termed LDL receptor-related protein (Lrp)13, were sequenced from striped bass ( Morone saxatilis ) and white perch ( Morone americana ) ovaries. Receptor proteins were purified from perch ovary membranes by protein-affinity chromatography employing an immobilized mixture of vitellogenins Aa and Ab. RT-PCR revealed lrp13 to be predominantly expressed in striped bass ovary, and in situ hybridization detected lrp13 transcripts in the ooplasm of early secondary growth oocytes. Quantitative RT-PCR confirmed peak lrp13 expression in the ovary during early secondary growth. Quantitative mass spectrometry revealed peak Lrp13 protein levels in striped bass ovary during late-vitellogenesis, and immunohistochemistry localized Lrp13 to the oolemma and zona radiata of vitellogenic oocytes. Previously unreported orthologs of lrp13 were identified in genome sequences of fishes, chicken ( Gallus gallus ), mouse ( Mus musculus ), and dog ( Canis lupus familiaris ). Zebrafish ( Danio rerio ) and Nile tilapia ( Oreochromis niloticus ) lrp13 loci are discrete and share genomic synteny. The Lrp13 appears to function as a vitellogenin receptor and may be an important mediator of yolk formation in fishes and other oviparous vertebrates. The presence of lrp13 orthologs in mammals suggests that this lipoprotein receptor is widely distributed among vertebrates, where it may generally play a role in lipoprotein metabolism.
关键词：affinity purification ; egg ; endocytosis ; oocyte ; oogenesis ; ovary ; vitellogenesis ; very low density lipoprotein receptor ; yolk ; low density lipoprotein receptor-related protein 13