文章基本信息

标题：Lipoprotein-apheresis reduces circulating microparticles in individuals with familial hypercholesterolemia
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作者：Katherine D. Connolly ; Gareth R. Willis ; Dev B. N. Datta 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2014
卷号：55
期号：10
页码：2064-2072
DOI：10.1194/jlr.M049726
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Lipoprotein-apheresis (apheresis) removes LDL-cholesterol in patients with severe dyslipidemia. However, reduction is transient, indicating that the long-term cardiovascular benefits of apheresis may not solely be due to LDL removal. Microparticles (MPs) are submicron vesicles released from the plasma membrane of cells. MPs, particularly platelet-derived MPs, are increasingly being linked to the pathogenesis of many diseases. We aimed to characterize the effect of apheresis on MP size, concentration, cellular origin, and fatty acid concentration in individuals with familial hypercholesterolemia (FH). Plasma and MP samples were collected from 12 individuals with FH undergoing routine apheresis. Tunable resistive pulse sensing (np200) and nanoparticle tracking analysis measured a fall in MP concentration (33 and 15%, respectively; P
关键词：extracellular vesicles ; microvesicles ; exosomes ; low density lipoprotein-apheresis ; phosphatidylserine ; nanoparticle tracking analysis ; tunable resistive pulse sensing ; flow cytometry ; fatty acids