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标题：Discovery of a linoleate 9S-dioxygenase and an allene oxide synthase in a fusion protein of Fusarium oxysporum
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作者：Inga Hoffmann ; Ernst H. Oliw
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2013
卷号：54
期号：12
页码：3471-3480
DOI：10.1194/jlr.M044347
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Fusarium oxysporum is a devastating plant pathogen that oxidizes C₁₈ fatty acids sequentially to jasmonates. The genome codes for putative dioxygenase (DOX)-cytochrome P450 (CYP) fusion proteins homologous to linoleate diol synthases (LDSs) and the allene oxide synthase (AOS) of Aspergillus terreus , e.g., FOXB_01332. Recombinant FOXB_01332 oxidized 18:2n-6 to 9 S -hydroperoxy-10( E ),12( Z )-octadecadienoic acid by hydrogen ion and antarafacial insertion of molecular oxygen and sequentially to an allene oxide, 9 S (10)-epoxy-10,12( Z )-octadecadienoic acid, as judged from nonenzymatic hydrolysis products (α- and γ-ketols). The enzyme was therefore designated 9 S -DOX-AOS. The 9 S -DOX activity oxidized C₁₈ and C₂₀ fatty acids of the n-6 and n-3 series to hydroperoxides at the n-9 and n-7 positions, and the n-9 hydroperoxides could be sequentially transformed to allene oxides with only a few exceptions. The AOS activity was stereospecific for 9- and 11-hydroperoxides with S configurations. FOXB_01332 has acidic and alcoholic residues, Glu⁹⁴⁶-Val-Leu-Ser⁹⁴⁹, at positions of crucial Asn and Gln residues (Asn-Xaa-Xaa-Gln) of the AOS and LDS. Site-directed mutagenesis studies revealed that FOXB_01332 and AOS of A. terreus differ in catalytically important residues suggesting that AOS of A. terreus and F. oxysporum belong to different subfamilies. FOXB_01332 is the first linoleate 9-DOX with homology to animal heme peroxidases and the first 9-DOX-AOS fusion protein.
关键词：cytochrome P450 class III ; heme peroxidase ; linoleate diol synthase ; mutagenesis site-specific ; oxylipin biosynthesis ; oxygenation mechanism