文章基本信息

标题：Alterations in cerebrospinal fluid glycerophospholipids and phospholipase A2 activity in Alzheimer's disease
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作者：Alfred N. Fonteh ; Jiarong Chiang ; Matthew Cipolla 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2013
卷号：54
期号：10
页码：2884-2897
DOI：10.1194/jlr.M037622
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Our aim is to study selected cerebrospinal fluid (CSF) glycerophospholipids (GP) that are important in brain pathophysiology. We recruited cognitively healthy (CH), minimally cognitively impaired (MCI), and late onset Alzheimer's disease (LOAD) study participants and collected their CSF. After fractionation into nanometer particles (NP) and supernatant fluids (SF), we studied the lipid composition of these compartments. LC-MS/MS studies reveal that both CSF fractions from CH subjects have N-acyl phosphatidylethanolamine, 1-radyl-2-acyl- sn -glycerophosphoethanolamine (PE), 1-radyl-2-acyl- sn -glycerophosphocholine (PC), 1,2-diacyl- sn -glycerophosphoserine (PS), platelet-activating factor-like lipids, and lysophosphatidylcholine (LPC). In the NP fraction, GPs are enriched with a mixture of saturated, monounsaturated, and polyunsaturated fatty acid species, while PE and PS in the SF fractions are enriched with PUFA-containing molecular species. PC, PE, and PS levels in CSF fractions decrease progressively in participants from CH to MCI, and then to LOAD. Whereas most PC species decrease equally in LOAD, plasmalogen species account for most of the decrease in PE. A significant increase in the LPC-to-PC ratio and PLA₂ activity accompanies the GP decrease in LOAD. These studies reveal that CSF supernatant fluid and nanometer particles have different GP composition, and that PLA₂ activity accounts for altered GPs in these fractions as neurodegeneration progresses.
关键词：lipid metabolism ; minimal cognitive impairment ; liquid chromatography ; mass spectrometry ; nanoparticles ; neurodegeneration