文章基本信息

标题：Chylomicron metabolism in rats: kinetic modeling indicates that the particles remain at endothelial sites for minutes
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作者：Magnus Hultin ; Roger Savonen ; Olivier Chevreuil 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2013
卷号：54
期号：10
页码：2595-2605
DOI：10.1194/jlr.M032979
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Chylomicrons labeled in vivo with ¹⁴C-oleic acid (primarily in triglycerides, providing a tracer for lipolysis) and ³H-retinol (primarily in ester form, providing a tracer for the core lipids) were injected into rats. Radioactivity in tissues was followed at a series of times up to 40 min and the data were analyzed by compartmental modeling. For heart-like tissues it was necessary to allow the chylomicrons to enter into a compartment where lipolysis is rapid and then transfer to a second compartment where lipolysis is slower. The particles remained in these compartments for minutes and when they returned to blood they had reduced affinity for binding in the tissue. In contrast, the data for liver could readily be fitted with a single compartment for native and lipolyzed chylomicrons in blood, and there was no need for a pathway back to blood. A composite model was built from the individual tissue models. This whole-body model could simultaneously fit all data for both fed and fasted rats and allowed estimation of fluxes and residence times in the four compartments; native and lipolyzed chylomicrons (“remnants”) in blood, and particles in the tissue compartments where lipolysis is rapid and slow, respectively.
关键词：adipose tissue ; compartmental modeling ; chylomicrons ; chylomicron remnants ; endothelium ; heart ; lipoprotein kinetics ; lipolysis and fatty acid metabolism ; lipoprotein lipase ; margination