文章基本信息

标题：Regulation of skeletal muscle lipolysis and oxidative metabolism by the co-lipase CGI-58
本地全文：下载
作者：Pierre-Marie Badin ; Camille Loubière ; Maarten Coonen 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2012
卷号：53
期号：5
页码：839-848
DOI：10.1194/jlr.M019182
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We investigated here the specific role of CGI-58 in the regulation of energy metabolism in skeletal muscle. We first examined CGI-58 protein expression in various muscle types in mice, and next modulated CGI-58 expression during overexpression and knockdown studies in human primary myotubes and evaluated the consequences on oxidative metabolism. We observed a preferential expression of CGI-58 in oxidative muscles in mice consistent with triacylglycerol hydrolase activity. We next showed by pulse-chase that CGI-58 overexpression increased by more than 2-fold the rate of triacylglycerol (TAG) hydrolysis, as well as TAG-derived fatty acid (FA) release and oxidation. Oppositely, CGI-58 silencing reduced TAG hydrolysis and TAG-derived FA release and oxidation (−77%, P
关键词：comparative gene identification 58 ; fatty acid ; substrate oxidation ; intramyocellular triacylglycerol ; peroxisome proliferator-activated receptor ; mitochondria