文章基本信息

标题：Adiposoft: automated software for the analysis of white adipose tissue cellularity in histological sections
本地全文：下载
作者：Miguel Galarraga ; Javier Campión ; Arrate Muñoz-Barrutia 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2012
卷号：53
期号：12
页码：2791-2796
DOI：10.1194/jlr.D023788
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The accurate estimation of the number and size of cells provides relevant information on the kinetics of growth and the physiological status of a given tissue or organ. Here, we present Adiposoft, a fully automated open-source software for the analysis of white adipose tissue cellularity in histological sections. First, we describe the sequence of image analysis routines implemented by the program. Then, we evaluate our software by comparing it with other adipose tissue quantification methods, namely, with the manual analysis of cells in histological sections (used as gold standard) and with the automated analysis of cells in suspension, the most commonly used method. Our results show significant concordance between Adiposoft and the other two methods. We also demonstrate the ability of the proposed method to distinguish the cellular composition of three different rat fat depots. Moreover, we found high correlation and low disagreement between Adiposoft and the manual delineation of cells. We conclude that Adiposoft provides accurate results while considerably reducing the amount of time and effort required for the analysis.
关键词：fat cell diameter ; fat tissue cellularity ; adipocyte ; quantification ; image analysis ; software validation ; obesity