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标题：MALDI imaging MS of phospholipids in the mouse lung
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作者：Karin A. Zemski Berry ; Bilan Li ; Susan D. Reynolds 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2011
卷号：52
期号：8
页码：1551-1560
DOI：10.1194/jlr.M015750
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Lipid mediators are important in lung biochemistry and are derived from the enzymatic oxidation of arachidonic and docosahexaenoic acids, which are PUFAs that are present in phospholipids in cell membranes. In this study, MALDI imaging MS was used to determine the localization of arachidonate- and docosahexaenoate-containing phospholipids in mouse lung. These PUFA-containing phospholipids were determined to be uniquely abundant at the lining of small and large airways, which were unequivocally identified by immunohistochemistry. In addition, it was found that the blood vessels present in the lung were characterized by sphingomyelin molecular species, and lung surfactant phospholipids appeared evenly distributed throughout the lung parenchyma, indicating alveolar localization. This technique revealed unexpected high concentrations of arachidonate- and docosahexaenoate-containing phospholipids lining the airways in pulmonary tissue, which could serve as precursors of lipid mediators affecting airways biology.
关键词：arachidonic acid ; lipid localization ; cryoembedding agent ; lipid mediators ; pulmonary tissue ; mass spectrometry