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标题：LC/MS analysis of stratum corneum lipids: ceramide profiling and discovery
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作者：Jeroen van Smeden ; Louise Hoppel ; Rob van der Heijden 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2011
卷号：52
期号：6
页码：1211-1221
DOI：10.1194/jlr.M014456
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Ceramides (CERs) in the upper layer of the skin, the stratum corneum (SC), play a key role in the skin barrier function. In human SC, the literature currently reports 11 CER subclasses that have been identified. In this paper, a novel quick and robust LC/MS method is presented that allows the separation and analysis of all known human SC CER subclasses using only limited sample preparation. Besides all 11 known and identified subclasses, a 3D multi-mass chromatogram shows the presence of other lipid subclasses. Using LC/MS/MS with an ion trap (IT) system, a Fourier transform-ion cyclotron resonance system, and a triple quadrupole system, we were able to identify one of these lipid subclasses as a new CER subclass: the ester-linked ω-hydroxy fatty acid with a dihydrosphingosine base (CER [EOdS]). Besides the identification of a new CER subclass, this paper also describes the applicability and robustness of the developed LC/MS method by analyzing three (biological) SC samples: SC from human dermatomed skin, human SC obtained by tape stripping, and SC from full-thickness skin explants. All three biological samples showed all known CER subclasses and slight differences were observed in CER profile.
关键词：liquid chromatography ; mass spectrometry ; sphingolipids ; human skin lipids ; normal-phase liquid chromatography ; atmospheric pressure chemical ionization mass spectrometry ; identification ; 3D multi-mass chromatogram