文章基本信息

标题：Measurement of serum PON-3 concentration: method evaluation, reference values, and influence of genotypes in a population-based study
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作者：Gerard Aragonès ; Marta Guardiola ; María Barreda 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2011
卷号：52
期号：5
页码：1055-1061
DOI：10.1194/jlr.D014134
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Experimental studies showed that paraoxonase-3 (PON3) retards lipoprotein oxidation. Our objective was to describe a new assay to measure serum PON3 concentrations and report their reference values in a population-based study. The influence of PON3 promoter polymorphisms and their relationships with PON1 and lipid profile were also studied. We generated an anti-PON3 antibody by inoculating rabbits with a synthetic peptide specific to mature PON3. This antibody was used to develop an ELISA. The average regression line of standard plots (n = 8) was y = 0.9587 (0.3392) log₁₀ x + 1.9466 (0.0861) [ r ² = 0.924 (0.0131); P
关键词：analytical methods ; antioxidants ; atherosclerosis ; lipoproteins ; oxidative stress