文章基本信息

标题：Biochemical studies on sphingolipids of Artemia franciscana: novel neutral glycosphingolipids
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作者：Hisao Kojima ; Takemasa Shimizu ; Mutsumi Sugita 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2011
卷号：52
期号：2
页码：308-317
DOI：10.1194/jlr.M010173
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Neutral glycosphingolipids containing one to six sugars in their oligosaccharide chains have been isolated from cysts of the brine shrimp Artemia franciscana . The structures of these glycolipids were identified by methylation analysis, partial acid hydrolysis, gas-liquid chromatography, combined gas-liquid chromatography-mass spectrometry, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and proton nuclear magnetic resonance spectroscopy to be Glcβ1-Cer, Manβ1-4Glcβ1-Cer, Fucα1-3Manβ1-4Glcβ1-Cer, GlcNAcβ1-3Manβ1-4Glcβ1-Cer, GlcNAcα1-2Fucα1-3Manβ1-4Glcβ1-Cer, GalNAcβ1-4GlcNAcβ1-3Manβ1-4Glcβ1-Cer, GalNAcβ1-4(Fucα1-3)GlcNAcβ1-3Manβ1-4Glcβ1-Cer (CPS), and GalNAcβ1-4(GlcNAcα1-2Fucα1-3)GlcNAcβ1-3Manβ1-4Glcβ1-Cer (CHS). Two glycosphingolipids, CPS and CHS, were characterized as novel structures. Because Artemia contains a certain series of glycosphingolipids (-Fucα3Manβ4GlcβCer), which differ from the core sugar sequences reported thus far, we tentatively designated the glycosphingolipids characterized as nonarthro-series ones. Furthermore, CHS exhibited a hybrid structure of arthro-series and nonarthro-series sugar chain. Two novel glycosphingolipids were characterized from the brine shrimp Artemia franciscana ; one was composed of arthrotetraose and a branching fucose attached to N -acetylglucosamine residue, and the other was composed of CPS with an additional N -acetylglucosamine residue attached to the branching fucose.
关键词：sphingolipid ; sphingosine ; long-chain base ; oligosaccharide ; fucomannolipid ; terminal alpha- N -acetylglucosamine residue ; structure characterization ; conventional destructive analysis ; Branchiopoda ; dormant cyst