文章基本信息

标题：Sphingolipidomics of A2780 human ovarian carcinoma cells treated with synthetic retinoids
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作者：Manuela Valsecchi ; Massimo Aureli ; Laura Mauri 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2010
卷号：51
期号：7
页码：1832-1840
DOI：10.1194/jlr.M004010
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The dihydroceramide, ceramide, sphingomyelin, lactosylceramide, and ganglioside species of A2780 human ovarian carcinoma cells treated with the synthetic retinoids N -(4-hydroxyphenyl)retinamide (fenretinide, 4-HPR) and 4-oxo- N -(4-hydroxyphenyl)retinamide (4-oxo-4-HPR) in culture were characterized by ESI-MS. We characterized 32 species of ceramide and dihydroceramide, 15 of sphingomyelin, 12 of lactosylceramide, 9 of ganglioside GM2, and 6 of ganglioside GM3 differing for the long-chain base and fatty acid structures. Our results indicated that treatment with both 4-HPR and 4-oxo-4-HPR led to a marked increase in dihydroceramide species, while only 4-oxo-4-HPR led to a minor increase of ceramide species. Dihydroceramides generated in A2780 cells in response to 4-HPR or 4-oxo-4-HPR differed for their fatty acid content, suggesting that the two drugs differentially affect the early steps of sphingolipid synthesis. Dihydroceramides produced upon treatments with the drugs were further used for the synthesis of complex dihydrosphingolipids, whose levels dramatically increased in drug-treated cells.
关键词：4-HPR ; fenretinide ; 4-oxo-4-HPR