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标题：Apo B100 similarities to viral proteins suggest basis for LDL-DNA binding and transfection capacity
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作者：Juan Guevara ; Nagindra Prashad ; Boris Ermolinsky 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2010
卷号：51
期号：7
页码：1704-1718
DOI：10.1194/jlr.M003277
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：LDL mediates transfection with plasmid DNA in a variety of cell types in vitro and in several tissues in vivo in the rat. The transfection capacity of LDL is based on apo B100, as arginine/lysine clusters, suggestive of nucleic acid-binding domains and nuclear localization signal sequences, are present throughout the molecule. Apo E may also contribute to this capacity because of its similarity to the Dengue virus capsid proteins and its ability to bind DNA. Synthetic peptides representing two apo B100 regions with prominent Arg/Lys clusters were shown to bind DNA. Region 1 (⁰⁰¹⁴Lys-Ser⁰¹⁶⁰) shares sequence motifs present in DNA binding domains of Interferon Regulatory Factors and Flaviviridae capsid/core proteins. It also contains a close analog of the B/E receptor ligand of apo E. Region 1 peptides, B1-1 (⁰⁰¹⁴Lys-Glu⁰⁰⁵⁴) and B1-2 (⁰⁰⁵⁵Leu-Ala⁰⁰⁹⁶), mediate transfection of HeLa cells but are cytotoxic. Region 2 (³³¹³Asp-Thr³⁴³¹), containing the known B/E receptor ligand, shares analog motifs with the human herpesvirus 5 immediate-early transcriptional regulator (UL122) and Flaviviridae NS3 helicases. Region 2 peptides, B2-1 (³³¹³Asp-Glu³³⁵⁵), and B2-2 (³³⁵⁶Gly-Thr³⁴³¹) are ineffective in cell transfection and are noncytotoxic. These results confirm the role of LDL as a natural transfection vector in vivo, a capacity imparted by the apo B100, and suggest a basis for Flaviviridae cell entry.
关键词：Flaviviridae capsid/core proteins ; synthetic peptides ; KXXK motif