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标题：Novel myelin penta- and hexa-acetyl-galactosyl-ceramides: structural characterization and immunoreactivity in cerebrospinal fluid
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作者：Maria Podbielska ; Somsankar Dasgupta ; Steven B. Levery 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2010
卷号：51
期号：6
页码：1394-1406
DOI：10.1194/jlr.M001396
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Fast migrating cerebrosides (FMC) are derivatives of galactosylceramide (GalCer). The structures of the most hydrophobic FMC-5, FMC-6, and FMC-7 were determined by electrospray ionization linear ion-trap mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy complementing previous NMR spectroscopy and gas chromatography-mass spectrometry to be 3- O -acetyl-sphingosine-GalCer derivatives with galactose O- acetyl modifications. FMC-5 and FMC-6 are 3- O- acetyl-sphingosine-2,3,4,6-tetra- O- acetyl-GalCer with nonhydroxy and hydroxy- N- fatty-acids, while FMC-7 has an additional O- acetylation of the 2-hydroxy-fatty acid. The immuno-reactivity in human cerebrospinal fluid (CSF) to these acetylated glycolipids was examined in central nervous system (CNS) infectious disease, noninflammatory disorders, and multiple sclerosis (MS). Screening for lipid binding in MS and other neurological disease groups revealed that the greatest anti-hydrophobic FMC reactivity was observed in the inflammatory CNS diseases (meningitis, meningo-encephalitis, and subacute sclerosing panencephalitis). Some MS patients had increased reactivity with the hydrophobic FMCs and with glycoglycerophospholipid MfGL-II from Mycoplasma fermentans . The cross-reactivity of highly acetylated GalCer with microbial acyl-glycolipid raises the possibility that myelin- O -acetyl-cerebrosides, bacterial infection, and neurological disease are linked.
关键词：acetyl-cerebroside ; cross-reactivity ; electrospray ionization ; Escherichia coli ; fast migrating cerebrosides ; glycosphingolipids ; inflammation ; lipopolysaccharide ; multiple sclerosis ; multistage mass spectrometry ; Mycoplasma fermentans ; neurological disease