文章基本信息

标题：VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation
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作者：Sarada D. Tetali ; Madhu S. Budamagunta ; Catalina Simion 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2010
卷号：51
期号：6
页码：1273-1283
DOI：10.1194/jlr.M000406
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Our previous work indicated that apolipoprotein (apo) E4 assumes a more expanded conformation in the postprandial period. The postprandial state is characterized by increased VLDL lipolysis. In this article, we tested the hypothesis that VLDL lipolysis products increase VLDL particle fluidity, which mediates expansion of apoE4 on the VLDL particle. Plasma from healthy subjects was collected before and after a moderately high-fat meal and incubated with nitroxyl-spin labeled apoE. ApoE conformation was examined by electron paramagnetic resonance spectroscopy using targeted spin probes on cysteines introduced in the N-terminal (S76C) and C-terminal (A241C) domains. Further, we synthesized a novel nitroxyl spin-labeled cholesterol analog, which gave insight into lipoprotein particle fluidity. Our data revealed that the order of lipoprotein fluidity was HDL∼LDL
关键词：lipid fluidity ; postprandial state ; structural conformation ; very low density lipoprotein