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标题：Marked elevations in pro-inflammatory polyunsaturated fatty acid metabolites in females with irritable bowel syndrome
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作者：Gerard Clarke ; Peter Fitzgerald ; Alan A. Hennessy 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2010
卷号：51
期号：5
页码：1186-1192
DOI：10.1194/jlr.P000695
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder referred to gastroenterologists. Although the pathophysiology remains unclear, accumulating evidence points to the presence of low-level immune activation both in the gut and systemically. Circulating polyunsaturated fatty acids (PUFA) have recently attracted attention as being altered in a variety of disease states. Arachidonic acid (AA), in particular, has been implicated in the development of a pro-inflammatory profile in a number of immune-related disorders. AA is the precursor of a number of important immunomodulatory eicosanoids, including prostaglandin E₂ (PGE₂) and leukotriene B₄ (LTB₄). We investigated the hypothesis that elevated plasma AA concentrations in plasma contribute to the proposed pro-inflammatory profile in IBS. Plasma AA and related PUFA were quantified by gas chromatography analysis in IBS patients and controls. Both PGE₂ and LTB₄ were measured in serum using commercially available ELISA assays. AA concentrations were elevated in our patient cohort compared with healthy controls. Moreover, we demonstrated that this disturbance in plasma AA concentrations leads to downstream elevations in eicosanoids. Together, our data identifies a novel proinflammatory mechanism in irritable bowel syndrome and also suggests that elevated arachidonic acid levels in plasma may serve as putative biological markers in this condition.
关键词：arachidonic acid ; biological marker ; eicosanoid ; gas chromatography ; IBS ; pro-inflammatory profile