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标题：Calpain-10 is a component of the obesity-related quantitative trait locus Adip1
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作者：James M. Cheverud ; Gloria L. Fawcett ; Joseph P. Jarvis 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2010
卷号：51
期号：5
页码：907-913
DOI：10.1194/jlr.M900128-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We previously mapped Adip1 , an obesity quantitative trait locus (QTL), to the central portion of murine chromosome 1 containing the calpain-10 ( Capn10 ) gene. Human studies have associated calpain-10 ( CAPN10 ) variants with type 2 diabetes and various metabolic traits. We performed a quantitative hybrid complementation test (QHCT) to determine whether differences attributed to Adip1 are the result of variant Capn10 alleles in LG/J and SM/J mice. We crossed LG/J and SM/J to wild-type (C57BL/6J) and Capn10 knockout ( Capn10 ^−/−) mice to form four F₁ hybrid groups: LG/J by wild-type, LG/J by Capn10 ^−/−, SM/J by wild-type, and SM/J by Capn10 ^−/−. We performed a two-way ANOVA with the experimental strain, tester strain, and their interaction as the factors. Significant interaction indicates a quantitative failure to complement. We found failure to complement for fat, organ, and body weights, and leptin, female free fatty acid, and triglyceride levels. Capn10 ^−/− resulted in heavier weights and higher serum levels in LG/J crosses but not in SM/J crosses. For glucose tolerance and insulin response tests, the Capn10 ^−/− allele resulted in lower glucose levels in crosses with SM/J but had no effect in the LG/J crosses. Differences between the LG/J and SM/J Capn10 alleles are the likely source of some of the QTL effects mapped to Adip1 in the LG/J–by–SM/J cross. Capn10 plays an important role in regulating obesity and diabetes in mice.
关键词：diabetes ; glucose tolerance ; leptin ; quantitative hybrid complementation test