文章基本信息

标题：Adiponectin does not bind to gelatin: a new and easy way to purify high-molecular-weight adiponectin from human plasma
本地全文：下载
作者：Yasuko Nakano ; Ayako Shoji ; Atsushi Arakawa 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2010
卷号：51
期号：1
页码：210-215
DOI：10.1194/jlr.D900010-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Human plasma contains three forms of adiponectin, a trimer, a hexamer, and a high-molecular-weight (HMW) multimer. We previously reported HMW adiponectin was a gelatin-binding protein of 28 kDa (GBP28), it having been purified due to its affinity to gelatin-Cellulofine (Nakano, Y., et al. Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma. J. Biochem . 1996. 120: 803–12). Although HMW adiponectin binds to gelatin-Cellulofine, it cannot bind to gelatin-Sepharose. Gelatin-Cellulofine was made of formyl-Cellulofine and gelatin, and we found that HMW adiponectin binds to reduced formyl-Cellulofine with similar affinity as to gelatin-Cellulofine. Through only two steps using reduced formyl-Cellulofine and DEAE-Sepharose, HMW adiponectin can be effectively purified from human plasma.
关键词：reduced formyl-Cellulofine ; gelatin-Cellulofine ; osteoclastogenesis