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标题：Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
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作者：Francisco J. Pérez-Cano ; Carolina Ramírez-Santana ; Marta Molero-Luís 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2009
卷号：50
期号：3
页码：467-476
DOI：10.1194/jlr.M800356-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The aim of this work was to establish the effect of the cis 9, trans 11 conjugated linoleic acid (CLA) isomer on mucosal immunity during early life in rats, a period when mucosal immunoglobulin production is poorly developed, as is also the case in humans. CLA supplementation was performed during three life periods: gestation, suckling, and early infancy. The immune status of supplemented animals was evaluated at two time points: at the end of the suckling period (21-day-old rats) and 1 week after weaning (28-day-old rats). Secretory IgA was quantified in intestinal washes from 28-day-old rats by ELISA technique. IgA, TGFβ, and PPARγ mRNA expression was measured in small intestine and colon by real time PCR, using Taqman® specific probes and primers. IgA mucosal production was enhanced in animals supplemented with CLA during suckling and early infancy: in 28-day-old rats, IgA mRNA expression was increased in small intestine and colon by approximately 6- and 4-fold, respectively, and intestinal IgA protein by ∼2-fold. TGFβ gene expression was independent of age and type of tissue considered, and was not modified by dietary CLA. Gene expression of PPARγ, a possible mediator of CLA's effects was also upregulated in animals receiving CLA during early life. In conclusion, dietary supplementation with CLA during suckling and extended to early infancy enhances development of the intestinal immune response in rats.