文章基本信息

标题：Spatial and temporal alterations of phospholipids determined by mass spectrometry during mouse embryo implantation
本地全文：下载
作者：Kristin E. Burnum ; Dale S. Cornett ; Satu M. Puolitaival 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2009
卷号：50
期号：11
页码：2290-2298
DOI：10.1194/jlr.M900100-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Molecular events involved in successful embryo implantation are not well understood. In this study, we used MALDI imaging mass spectrometry (IMS) technologies to characterize the spatial and temporal distribution of phospholipid species associated with mouse embryo implantation. Molecular images showing phospholipid distribution within implantation sites changed markedly between distinct cellular areas during days 4–8 of pregnancy. For example, by day 8, linoleate- and docosahexaenoate-containing phospholipids localized to regions destined to undergo cell death, whereas oleate-containing phospholipids localized to angiogenic regions. Arachidonate-containing phospholipids showed different segregation patterns depending on the lipid class, revealing a strong correlation of phosphatidylethanolamines and phosphatidylinositols with cytosolic phospholipase A_2α and cyclooxygenase-2 during embryo implantation. LC-ESI-MS/MS was used to validate MALDI IMS phospholipid distribution patterns. Overall, molecular images revealed the dynamic complexity of lipid distributions in early pregnancy, signifying the importance of complex interplay of lipid molecules in uterine biology and implantation.
关键词：imaging mass spectrometry ; phosphatidylcholine ; sphingomyelins ; phosphatidylinositol ; phosphatidylserine ; phosphatidylglycerol ; phosphatidylethanolamine