文章基本信息

标题：The structure of plasmalogens: VII. Analysis of mammalian liver lipids and the interference of vitamin A in the analysis
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作者：German Camejo ; Maurice M. Rapport ; Gene A. Morrill 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1964
卷号：5
期号：1
页码：75-80
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Low values of the molar ratio of α,β-unsaturated ether to aldehydogenic lipid found with lipid extracts of mouse and rat liver are shown to result solely from the presence in these extracts of vitamin A rather than of long-chain aldehydes or mixed acetals. Vitamin A interferes in the spectrophotometric determination of specific iodination of α,β-unsaturated ethers because it absorbs some light at 355 mμ. The correction is made simply by using an appropriate sample blank. Vitamin A interferes much more seriously with the estimation of aldehydogenic lipids as p -nitrophenylhydrazones because of its transformation under the reaction conditions to anhydro vitamin A. The correction for this interference may be made by independent estimation of the vitamin A content (Carr-Price reaction), determining a standard curve for vitamin A subjected to the p -nitrophenylhydrazone procedure, and subtracting the appropriate value. The vitamin A content of liver is dependent on the species of animal as well as on diet. In the animals studied, the average level of interference of vitamin A in estimations of liver plasmalogen was 75% for the mouse, 50% for the rat, 8% for the rabbit, and negligible for guinea pig.