文章基本信息

标题：Analysis of low-density lipoproteins by preparative ultracentrifugation and refractometry
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作者：F. T. Lindgren ; A. V. Nichols ; N. K. Freeman 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1964
卷号：5
期号：1
页码：68-74
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A simplified method for the analysis of both the glyceride-rich S_f 20-10⁵ and the cholesterol-rich S_f 0-20 low-density lipoproteins is presented. It consists of serum lipoprotein fractionation by preparative ultracentrifugation and subsequent quantitative analysis by refractometry. Comparison of this technique with the technically more difficult analytic ultracentrifugal methodology reveals comparable results for these two principal low-density lipoprotein groups. The relationships between these lipoproteins and the serum levels of total lipids, glycerides, total cholesterol, and phospholipids are also presented. One of the advantages of this procedure is that it provides a reliable and reproducible means for quantifying the principal glyceride-bearing lipoprotein group–the S_f 20-10⁵ lipoproteins.