文章基本信息

标题：Effect of lipid-bound apoA-I cysteine mutants on lipopolysaccharide-induced endotoxemia in mice
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作者：Yunlong Wang ; Xuewei Zhu ; Gang Wu 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2008
卷号：49
期号：8
页码：1640-1645
DOI：10.1194/jlr.M700446-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：HDL has been shown to be able to neutralize the toxicity of lipopolysaccharide (LPS). Our previous study ( J. Lipid Res. 2005. 46: 1303–1311) characterized the properties of secondary structure and in vitro functions of different cysteine mutants of apolipoprotein A-I. Here, we reconstituted recombinant HDLs (named rHDLwt, rHDL52, rHDL74, rHDL107, rHDL129, rHDL173, rHDL195, and rHDL228) by mixing wild type or those mutants with dipalmitoyl phosphatidylcholine and examined their in vivo effects on LPS-induced endotoxemia in mice. Our results showed that 24 h after injection, mice receiving rHDL74 or rHDL52 had a significant decrease of plasma tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β), compared with control mice receiving either saline or rHDLwt ( P