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标题：The common rs9939609 gene variant of the fat mass- and obesity-associated gene FTO is related to fat cell lipolysis
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作者：Kerstin Wåhlén ; Eva Sjölin ; Johan Hoffstedt 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2008
卷号：49
期号：3
页码：607-611
DOI：10.1194/jlr.M700448-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We investigated the rs9939609 single nucleotide polymorphism of the FTO gene in relation to fat cell function and adipose tissue gene expression in 306 healthy women with a wide range in body mass index (18–53 kg/m²). Subcutaneous adipose tissue biopsies were taken for fat cell metabolism studies and in a subgroup (n = 90) for gene expression analyses. In homozygous carriers of the T-allele, the in vitro basal (spontaneous) adipocyte glycerol release was increased by 22% ( P = 0.007) and the in vivo plasma glycerol level was increased by ∼30% ( P = 0.037) compared with carriers of the A allele. In contrast, there were no genotype effects on catecholamine-stimulated lipolysis or basal or insulin-induced lipogenesis. We found no difference between genotypes for adipose tissue mRNA levels of FTO, hormone-sensitive lipase, adipose triglyceride lipase, perilipin, or CGI-58. Finally, the adipose tissue level of FTO mRNA was increased in obesity ( P = 0.002), was similar in subcutaneous and omental adipose tissue, was higher in fat cells than in fat tissue ( P = 0.0007), and was induced at an early stage in the differentiation process ( P = 0.004). These data suggest a role of the FTO gene in fat cell lipolysis, which may be important in explaining why the gene is implicated in body weight regulation.