文章基本信息

标题：Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging
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作者：Nora Tahallah ; Alain Brunelle ; Sabine De La Porte 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2008
卷号：49
期号：2
页码：438-454
DOI：10.1194/jlr.M700421-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Human striated muscle samples, from male control and Duchenne muscular dystrophy-affected children, were subjected to cluster-time-of-flight secondary ion mass spectrometry (cluster-ToF-SIMS) imaging using a 25 keV Bi₃⁺ liquid metal ion gun under static SIMS conditions. Spectra and ion density maps, or secondary ion images, were acquired in both positive and negative ion mode over several areas of 500 × 500 μm² (image resolution, 256 × 256 pixels). Characteristic distributions of various lipids were observed. Vitamin E and phosphatidylinositols were found to concentrate within the cells, whereas intact phosphocholines accumulated over the most damaged areas of the dystrophic muscles, together with cholesterol and sphingomyelin species. Fatty acyl chain composition varied depending on the region, allowing estimation of the local damage extent.