文章基本信息

标题：siRNA screening reveals JNK2 as an evolutionary conserved regulator of triglyceride homeostasis
本地全文：下载
作者：Vinciane Grimard ; Julia Massier ; Doris Richter 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2008
卷号：49
期号：11
页码：2427-2440
DOI：10.1194/jlr.M800168-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Lipid homeostasis is essential for proper function of cells and organisms. To unravel new regulators of this system, we developed a screening procedure, combining RNA interference in HeLa cells and TLC, which enabled us to monitor modifications of lipid composition resulting from short, interfering RNA knock-downs. We applied this technique to the analysis of 600 human kinases. Despite the occurrence of off-target effects, we identified JNK2 as a new player in triglyceride (TG) homeostasis and lipid droplet metabolism and, more specifically, in the regulation of lipolysis. Similar control of the level of TGs and lipid droplets was observed for its Schizosaccharomyces pombe homolog, Sty1, suggesting an evolutionary conserved function of mitogen-activated protein kinases in the regulation of lipid storage in eukaryotic cells.