文章基本信息

标题：Initial interaction of apoA-I with ABCA1 impacts in vivo metabolic fate of nascent HDL
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作者：Anny Mulya ; Ji-Young Lee ; Abraham K. Gebre 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2008
卷号：49
期号：11
页码：2390-2401
DOI：10.1194/jlr.M800241-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We investigated the in vivo metabolic fate of pre-β HDL particles in human apolipoprotein A-I transgenic ( hA-I ^Tg) mice. Pre-β HDL tracers were assembled by incubation of [¹²⁵I]tyramine cellobiose-labeled apolipoprotein A-I (apoA-I) with HEK293 cells expressing ABCA1. Radiolabeled pre-β HDLs of increasing size (pre-β1, -2, -3, and -4 HDLs) were isolated by fast-protein liquid chromatography and injected into hA-I ^Tg-recipient mice, after which plasma decay, in vivo remodeling, and tissue uptake were monitored. Pre-β2, -3, and -4 had similar plasma die-away rates, whereas pre-β1 HDL was removed 7-fold more rapidly. Radiolabel recovered in liver and kidney 24 h after tracer injection suggested increased ( P