文章基本信息

标题：Leukocyte-derived hepatic lipase increases HDL and decreases en face aortic atherosclerosis in LDLr−/− mice expressing CETP
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作者：Neil J. Hime ; Audrey S. Black ; Josh J. Bulgrien 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2008
卷号：49
期号：10
页码：2113-2123
DOI：10.1194/jlr.M700564-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：In addition to hepatic expression, cholesteryl ester transfer protein (CETP) and hepatic lipase (HL) are expressed by human macrophages. The combined actions of these proteins have profound effects on HDL structure and function. It is not known how these HDL changes influence atherosclerosis. To elucidate the role of leukocyte-derived HL on atherosclerosis in a background of CETP expression, we studied low density lipoprotein receptor-deficient mice expressing human CETP (CETPtgLDLr^−/−) with a leukocyte-derived HL deficiency (HL^−/− BM). HL^−/− bone marrow (BM), CETPtgLDLr^−/− mice were generated via bone marrow transplantation. Wild-type bone marrow was transplanted into CETPtgLDLr^−/− mice to generate HL^+/+ BM, CETPtgLDLr^−/− controls. The chimeras were fed a high-fat, high-cholesterol diet for 14 weeks to promote atherosclerosis. In female HL^−/− BM, CETPtgLDLr^−/− mice plasma HDL-cholesterol concentration during high-fat feeding was decreased 27% when compared with HL^+/+ BM, CETPtgLDLr^−/− mice ( P en face aortic atherosclerosis ( P −/− mice, leukocyte-derived HL deficiency was associated with a 16% decrease in plasma HDL-cholesterol concentration and a 25% increase in aortic atherosclerosis. Thus, leukocyte-derived HL in CETPtgLDLr^−/− mice has an atheroprotective role that may involve increased HDL levels.