出版社：American Society for Biochemistry and Molecular Biology
摘要：We quantified the rates of incorporation of α-linolenic acid (α-LNA; 18:3n-3) into “stable” lipids (triacylglycerol, phospholipid, cholesteryl ester) and the rate of conversion of α-LNA to docosahexaenoic acid (DHA; 22: 6n-3) in the liver of awake male rats on a high-DHA-containing diet after a 5-min intravenous infusion of [1-14C]α-LNA. At 5 min, 72.7% of liver radioactivity (excluding unesterified fatty acid radioactivity) was in stable lipids, with the remainder in the aqueous compartment. Using our measured specific activity of liver α-LNA-CoA, in the form of the dilution coefficient λα-LNA-CoA, we calculated incorporation rates of unesterified α-LNA into liver triacylglycerol,phospholipid, and cholesteryl ester as 2,401, 749, and 9.6 nmol/s/g × 10−4, respectively, corresponding to turnover rates of 3.2, 8.7, and 2.9%/min and half-lives of 8–24 min. A lower limit for the DHA synthesis rate from α-LNA equaled 15.8 nmol/s/g × 10−4 (0.5% of the net in corporation rate). Thus, in rats on a high-DHA-containing diet, rates of β-oxidation and esterification of α-LNA into stable liver lipids are high, whereas its conversion to DHA is comparatively low and insufficient to supply significant DHA to the brain. High incorporation and turnover rates likely reflect a high secretion rate by liver of stable lipids within very low density lipoproteins.
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