文章基本信息

标题：Storage of human plasma samples leads to alterations in the lipoprotein distribution of apoC-III and apoE
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作者：Jeffrey S. Cohn ; Claudia Rodriguez ; Hélène Jacques 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2004
卷号：45
期号：8
页码：1572-1579
DOI：10.1194/jlr.D300041-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The effect of frozen storage on lipoprotein distribution of apolipoprotein C-III (apoC-III) and apoE was investigated by measuring apoC-III and apoE by ELISA in HDL and apoB-containing lipoproteins of human plasma samples (n = 16) before and after 2 weeks of frozen storage (−20°C). HDLs were separated by heparin-manganese precipitation (HMP) or by fast-protein liquid chromatography (FPLC). Total plasma apoC-III and apoE levels were not affected by frozen storage. HDL-HMP apoC-III and apoE levels were significantly higher in frozen versus fresh samples: 7.7 ± 0.7 versus 6.7 ± 0.7 mg/dl ( P P P P Our results demonstrate that lipoprotein distribution of apoC-III and apoE is affected by storage of human plasma, suggesting that analysis of frozen plasma should be avoided in studies relating lipoprotein levels of apoC-III and/or apoE to the incidence of coronary artery disease.