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标题：Formation and antiproliferative effect of prostaglandin E3 from eicosapentaenoic acid in human lung cancer cells
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作者：Peiying Yang ; Diana Chan ; Edward Felix 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2004
卷号：45
期号：6
页码：1030-1039
DOI：10.1194/jlr.M300455-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We investigated the formation and pharmacology of prostaglandin E₃ (PGE₃) derived from fish oil eicosapentaenoic acid (EPA) in human lung cancer A549 cells. Exposure of A549 cells to EPA resulted in the rapid formation and export of PGE_3. The extracellular ratio of PGE₃ to PGE₂ increased from 0.08 in control cells to 0.8 in cells exposed to EPA within 48 h. Incubation of EPA with cloned ovine or human recombinant cyclooxygenase 2 (COX-2) resulted in 13- and 18-fold greater formation of PGE₃, respectively, than that produced by COX-1. Exposure of A549 cells to 1 μM PGE₃ inhibited cell proliferation by 37.1% ( P 3, however, had no effect. When A549 cells were exposed to EPA (25 μM) or a combination of EPA and celecoxib (a selective COX-2 inhibitor), the inhibitory effect of EPA on the growth of A549 cells was reversed by the presence of celecoxib (at both 5 and 10 μM). This effect appears to be associated with a 50% reduction of PGE₃ formation in cells treated with a combination of EPA and celecoxib compared with cells exposed to EPA alone. These data indicate that exposure of lung cancer cells to EPA results in a decrease in the COX-2-mediated formation of PGE₂, an increase in the level of PGE₃, and PGE₃-mediated inhibition of tumor cell proliferation.