文章基本信息

标题：Combined data from LDL composition and size measurement are compatible with a discoid particle shape
本地全文：下载
作者：Tom Teerlink ; Peter G. Scheffer ; Stephan J. L. Bakker 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2004
卷号：45
期号：5
页码：954-966
DOI：10.1194/jlr.M300521-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The size of LDL is usually reported as particle diameter, with the implicit assumption that it is a spherical particle. On the other hand, data obtained by cryoelectron microscopy and crystallographic analysis suggest that LDL shape may be discoid. We have investigated LDL particle geometry by combining data on LDL lipid composition with size measurement. The mean LDL diameter of 160 samples was measured by high-performance gel-filtration chromatography (HPGC), and particle volume was calculated from its lipid composition. Assuming a spherical shape, diameters calculated from volume correlated poorly with values obtained by HPGC ( R ² = 0.36). Assuming a discoid shape, particle height was calculated from volume and HPGC diameter. Diameter (20.9 ± 0.5 nm) and height (12.1 ± 0.8 nm) were not significantly related to each other ( r = 0.14, P = 0.09) and accounted for 23% and 77%, respectively, of the variation in particle volume. In multivariate regression models, LDL core lipids were the main determinants of height ( R ² = 0.83), whereas free cholesterol in the shell, which contributes only 5–9% to LDL mass, was the main determinant of diameter ( R ² = 0.54). We conclude that combined data from composition and size measurements are compatible with a discoid particle shape and propose a structural model for LDL in which free cholesterol plays a major role in determining particle shape and diameter.