文章基本信息

标题：A method of direct measurement for the enzymatic determination of cholesteryl esters
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作者：Toshimi Mizoguchi ; Toshiyuki Edano ; Tomoyuki Koshi 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2004
卷号：45
期号：2
页码：396-401
DOI：10.1194/jlr.D300024-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A direct measurement method for the enzymatic determination of cholesteryl esters (CEs) without measuring total cholesterol (TC) and free cholesterol (FC) is described. In the first step, hydrogen peroxide generated by cholesterol oxidase from FC was decomposed by catalase. In the second step, CE was measured by enzymatic determination using a colorimetric method or a fluorometric method. The measurement sensitivity of the fluorometric method was more than 20 times that of the colorimetric method. Optimal conditions of the assay were determined, and examples of measured CE in human plasma, rat liver, and cultured cells are indicated. The method of directly measuring CE was simple and has exceptional reproducibility compared with the technique of subtracting FC from TC using each measured TC and FC.