文章基本信息

标题：Rosiglitazone upregulates caveolin-1 expression in THP-1 cells through a PPAR-dependent mechanism
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作者：Gemma Llaverias ; Manuel Vázquez-Carrera ; Rosa M. Sánchez 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2004
卷号：45
期号：11
页码：2015-2024
DOI：10.1194/jlr.M400049-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Peroxisome proliferator-activated receptor γ (PPARγ) activation or overexpression induces caveolin-1 (cav-1) expression in several cell types. The objective of this study was to investigate if PPAR agonists could also regulate the cav-1 gene in macrophages and to explore the mechanisms involved. Our experiments demonstrated that rosiglitazone dose- and time-dependently increased cav-1 mRNA and protein in THP-1 macrophages. This induction was not observed in the presence of inhibitors of transcription or de novo protein synthesis. We also showed that the increase in cav-1 elicited by rosiglitazone was not related either to macrophage differentiation or to cellular apoptosis. The inductive effect seems to be dependent on PPAR activation, as the PPAR antagonist GW9662 abolished it. The activation of the liver X receptor with 22( R )-hydroxycholesterol also increased cav-1 mRNA, whereas the inactive ( S ) isomer did not. Finally, we identified a functional peroxisome proliferator response element in the cav-1 promoter that was activated upon rosiglitazone treatment in THP-1 macrophages.