文章基本信息

标题：Calcium-independent phospholipase A2 mediates CREB phosphorylation in double-stranded RNA-stimulated endothelial cells
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作者：Bradley D. Martinson ; Carolyn J. Albert ; John A. Corbett 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2003
卷号：44
期号：9
页码：1686-1691
DOI：10.1194/jlr.M300018-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：One of the products of a calcium-independent phospholipase A₂ (iPLA₂) attack of plasmenylcholine, lysoplasmenylcholine, has previously been shown to activate cAMP-dependent protein kinase (PKA). Because endothelial cells respond to some agonists in part by the activation of iPLA₂, the present study was designed to determine whether double-stranded RNA (dsRNA), the primary activator of the antiviral response in endothelial cells, elicits cAMP response element binding protein (CREB) phosphorylation through a mechanism mediated by iPLA₂. dsRNA stimulated CREB phosphorylation in bovine pulmonary artery endothelial cells that was inhibited by the iPLA₂ inhibitor, bromoenol lactone, and the PKA inhibitor, H-89. Additionally, the product of iPLA₂ hydrolysis of plasmenylcholine and lysoplasmenylcholine elicited CREB phosphorylation in bovine pulmonary endothelial cells. Taken together, the present studies suggest that dsRNA as well as other agonists of endothelial cells elicit signaling mechanisms that include in part CREB phosphorylation mediated by iPLA₂.