文章基本信息

标题：Analytical performance of a sandwich enzyme immunoassay for preβ1-HDL in stabilized plasma
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作者：Takashi Miida ; Osamu Miyazaki ; Yasushi Nakamura 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2003
卷号：44
期号：3
页码：645-650
DOI：10.1194/jlr.D200025-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We have established an immunoassay for preβ1-HDL (the initial acceptor of cellular cholesterol) using a monoclonal antibody, MAb55201. Because preβ1-HDL is unstable during storage, fresh plasma must be used for preβ1-HDL measurements. In this study, we describe a method of stabilizing preβ1-HDL, and evaluate the analytical performance of the immunoassay for preβ1-HDL. Fresh plasma was stored under various conditions with or without a pretreatment consisting of a 21-fold dilution into 50% (v/v) sucrose. Preβ1-HDL concentration was measured by immunoassay. In nonpretreated samples, preβ1-HDL decreased significantly from the baseline after 6 h at room temperature. Although preβ1-HDL was more stable at 0°C than at room temperature, it increased from 30.2 ± 8.5 (SE) to 56.5 ± 5.5 mg/l apolipoprotein A-I (apoA-I) ( P P P r = 0.833, P An immunoassay using MAb55201 with pretreated plasma is useful for clinical measurement of preβ1-HDL.