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标题：Studies on the metabolic fate of n-3 polyunsaturated fatty acids
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作者：Sacha Ferdinandusse ; Simone Denis ; Georges Dacremont 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2003
卷号：44
期号：10
页码：1992-1997
DOI：10.1194/jlr.M300223-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Several different processes involved in the metabolic fate of docosahexaenoic acid (DHA, C22:6n-3) and its precursor in the biosynthesis route, C24:6n-3, were studied. In cultured skin fibroblasts, the oxidation rate of [1-¹⁴C] 24:6n-3 was 2.7 times higher than for [1-¹⁴C]22:6n-3, whereas [1-¹⁴C]22:6n-3 was incorporated 7 times faster into different lipid classes than was [1-¹⁴C]24:6n-3. When determining the peroxisomal acyl-CoA oxidase activity, similar specific activities for C22:6(n-3)-CoA and C24:6(n-3)-CoA were found in mouse kidney peroxisomes. Thioesterase activity was measured for both substrates in mouse kidney peroxisomes as well as mitochondria, and C22:6(n-3)-CoA was hydrolyzed 1.7 times faster than C24:6(n-3)-CoA. These results imply that the preferred metabolic fate of C24:6(n-3)-CoA, after its synthesis in the endoplasmic reticulum (ER), is to move to the peroxisome, where it is β-oxidized, producing C22:6(n-3)-CoA. This DHA-CoA then preferentially moves back, probably as free fatty acid, to the ER, where it is incorporated into membrane lipids.