文章基本信息

标题：Brain lipid metabolism in the cPLA2 knockout mouse
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作者：Thad A. Rosenberger ; Nelly E. Villacreses ; Miguel A. Contreras 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2003
卷号：44
期号：1
页码：109-117
DOI：10.1194/jlr.M200298-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We examined brain phospholipid metabolism in mice in which the cytosolic phospholipase A₂ (cPLA_2, Type IV, 85 kDa) was knocked out (cPLA₂^−/− mice). Compared with controls, these mice demonstrated altered brain concentrations of several phospholipids, reduced esterified linoleate, arachidonate, and docosahexaenoate in choline glycerophospholipid, and reduced esterified arachidonate in phosphatidylinositol. Unanesthetized cPLA₂^−/− mice had reduced rates of incorporation of unlabeled arachidonate from plasma and from the brain arachidonoyl-CoA pool into ethanolamine glycerophospholipid and choline glycerophospholipid, but elevated rates into phosphatidylinositol. These differences corresponded to altered turnover and metabolic loss of esterified brain arachidonate. These results suggests that cPLA₂ is necessary to maintain normal brain concentrations of phospholipids and of their esterified polyunsaturated fatty acids. Reduced esterified arachidonate and docosahexaenoate may account for the resistance of the cPLA₂^−/− mouse to middle cerebral artery occlusion, and should influence membrane fluidity, neuroinflammation, signal transduction, and other brain processes.