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标题：Niemann-Pick C1 protein regulates cholesterol transport to the trans-Golgi network and plasma membrane caveolae
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作者：William S. Garver ; Kumar Krishnan ; Jayme R. Gallagos 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2002
卷号：43
期号：4
页码：579-589
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The Niemann-Pick C1 (NPC1) protein regulates cholesterol transport from late endosomes-lysosomes to other intracellular compartments. In this article, cholesterol transport to caveolin-1 and caveolin-2 containing compartments, such as the trans -Golgi network (TGN) and plasma membrane caveolae, was examined in normal (NPC^+/+), NPC heterozygous (NPC^+/−), and NPC homozygous (NPC^m/−) human fibroblasts. The expression and distribution of NPC1 in each cell type were similar, and characterized by a finely dispersed, granular staining pattern. The expression of caveolin-1 and caveolin-2 was increased in NPC^+/− and NPC^−/− fibroblasts, although the distribution in each cell type was similar and characterized by predominant staining of the TGN and plasma membrane. The TGN in NPC^+/+ fibroblasts was relatively cholesterol-enriched, whereas the TGN in NPC^+/− and NPC^−/− fibroblasts was partially or completely cholesterol-deficient, respectively. Consistent with studies demonstrating the transport of cholesterol from the TGN to plasma membrane caveolae, the concentration of cholesterol in plasma membrane caveolae isolated from NPC^+/− and NPC^−/− fibroblasts was significantly decreased, even though the total concentration of plasma membrane cholesterol in each cell type was similar. These studies demonstrate that NPC1 regulates cholesterol transport to caveolin-1 and caveolin-2 containing compartments such as the TGN and plasma membrane caveolae. —Garver, W. S., K. Krishnan, J. R. Gallagos, M. Michikawa, G. A. Francis, and R. A. Heidenreich. Niemann-Pick C1 protein regulates cholesterol transport to the trans -Golgi network and plasma membrane caveolae. J. Lipid Res. 2002. 43: 579–589.
关键词：caveolin-1 ; caveolin-2 ; fibroblasts