文章基本信息

标题：Familial combined hyperlipidemia plasma stimulates protein secretion by HepG2 cells identification of fibronectin in the differential secretion proteome
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作者：Marleen M. J. van Greevenbroek ; Vicky M. M-J. Vermeulen ; Tjerk W. A. de Bruin 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2002
卷号：43
期号：11
页码：1846-1854
DOI：10.1194/jlr.M100441-JLR200
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The aim of this study was to evaluate whether soluble factors in plasma of familial combined hyperlipidemia (FCHL) patients affect hepatic protein secretion. Cultured human hepatocytes, i.e., HepG2 cells, were incubated with fasting plasma (20%, v/v, in DMEM) from untreated FCHL patients or normolipidemic controls. Overall protein secretion was 10–15% higher after incubation with FCHL plasma. This was specifically caused by an increase in four secreted proteins, with estimated sizes of 240, 180, 120, and P P P P r = 0.61, P P FCHL plasma did not induce excess secretion of apoB by HepG2 cells compared with control plasma. FCHL plasma did stimulate secretion of several distinct hepatic proteins, among which fibronectin was identified.