文章基本信息

标题：ApoA-I secretion from HepG2 cells: evidence for the secretion of both lipid-poor apoA-I and intracellularly assembled nascent HDL
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作者：Jeffrey W. Chisholm ; Ellen R. Burleson ; Gregory S. Shelness 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2002
卷号：43
期号：1
页码：36-44
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The goal of this study was to determine whether apolipoprotein A-I (apoA-I) is lipidated before secretion by HepG2 cells. ApoA-I was extracted from microsomes after radiolabeling with [³⁵S]Met/Cys. After ultracentrifugal flotation, d 1.25 g/ml fractions were immunoprecipitated and analyzed by SDS-PAGE. Under steady state radiolabeling conditions, 20% of extracted microsomal apoA-I floated at d —Chisholm, J. W., E. R. Burleson, G. S. Shelness, and J. S. Parks. ApoA-I secretion from HepG2 cells: evidence for the secretion of both lipid-poor apoA-I and intracellularly assembled nascent HDL. J. Lipid Res. 2002. 43: 36–44.
关键词：apolipoprotein A-I ; carbonate extraction ; HDL metabolism ; HDL secretion ; HDL biosynthesis ; human ; lipid-free apoA-I ; lipid-poor apoA-I ; ultracentrifugation